Journal: Environmental Science. Nano

Article Title: Visualization of pore water colloids in intact soil using a new diffusive gradients in thin films (DGT)-based approach

doi: 10.1039/d5en01069a

Figure Lengend Snippet: FlFFF fractograms of the FYM pore water sample showing elemental signals (a–e) and the UV signal (f) as a function of colloid retention time ( T r ), which corresponds to increasing colloid size. Organic colloids (<5 nm) elute at T r < 300 s, while larger mineral colloids (5–450 nm) elute at T r > 300 s. The red line in (a) shows the molar Si/Al ratio of the mineral colloids, the dotted red line indicates the Si/Al ratio of the clay fraction isolated from the soil. In the largest size fraction ( T r > 3700 s), the Si/Al ratio (2.1), matches that of the isolated clay. The blue line in (b) shows the molar Fe/Al ratio of the mineral colloids, the dotted blue line the ratio of the isolated clay. The Fe/Al ratio in the mineral colloids is only slightly higher than in the isolated clay.

Article Snippet: The colloids in pore waters were characterised with field flow fractionation (FlFFF) (AF2000, Postnova Analytics) online coupled with a UV/vis detector (SPD-20A Postnova Analytics) and with ICP-MS with a previously described method, further instrumental details are in Section S3.

Techniques: Isolation

Journal: Biomacromolecules

Article Title: Biodegradable “Smart” Polyphosphazenes with Intrinsic Multifunctionality as Intracellular Protein Delivery Vehicles

doi: 10.1021/acs.biomac.7b00537

Figure Lengend Snippet: Figure 4. Non-covalent loading of protein on polyphosphazene carriers. (A) Avidin bound to polyphosphazenes as a percent of total protein in formulation and number of protein molecules per polymer chain (AF4 analysis, 210 nm, 0.015 mg/mL of polyphosphazene, 0.10 mg/mL of avidin, PBS, pH 7.4, cross- flow 4 mL/min); (B) AF4 profiles of avidin-polyphosphazene-FITC-biotin complexes resulting from (1) avidin-

Article Snippet: Asymmetric Flow Field Flow Fractionation, AF4 was performed using a Postnova AF2000 MT series (Postnova Analytics GmbH, Landsberg, Germany).

Techniques: Avidin-Biotin Assay, Formulation, Polymer

Journal: Environmental Science. Nano

Article Title: Visualization of pore water colloids in intact soil using a new diffusive gradients in thin films (DGT)-based approach

doi: 10.1039/d5en01069a

Figure Lengend Snippet: Left: Al accumulation on hydrogels with Zr–Chelex (referred to as DGT) and without (referred to as DET) for devices deployed in an isolated clay suspension and in a 1 mM CaCl 2 soil extract. Values represent means ± standard deviation from LA-ICP-MS line scans on 1 gel replicate. The BL was able to accumulate clay colloids because Al is higher in DGT versus corresponding DET samples. The truly dissolved Al contribution for DET and DGT is indicated and was estimated based on suspension composition and deployment time (details in Section S12). Right: SEM image of the dried BL after deployment in the isolated clay suspension (<0.2 μm fraction), the red arrow points to retained clay colloids in the gel matrix.

Article Snippet: The colloids in pore waters were characterised with field flow fractionation (FlFFF) (AF2000, Postnova Analytics) online coupled with a UV/vis detector (SPD-20A Postnova Analytics) and with ICP-MS with a previously described method, further instrumental details are in Section S3.

Techniques: Isolation, Suspension, Standard Deviation